Week 2: September 19, 2022 - September 23, 2022


September 20, 2022 

Set up for Lower Kanamycin Concentration Testing 

Since D.aquaticus only showed growth with Kanamycin, we set up to test five lower concentrations of Kanamyacin. First we made 250mL of TGY by combining tryptone, yeast extract, dextrose, and distilled water. It went in the autoclave overnight and the next day, the concentrations were prepared. There was two samples of each concentration in the end. Concentration one contained 3.125 μL/mL of antibiotic. Concentration two contained 2.733 μL/mL of antibiotic. Concentration three contained 2.34 μL/mL of antibiotic. Concentration four contained 1.95 μL/mL of antibiotic. The last concentration, number five, contained 1.56 μL/mL of antibiotic. Control one contained 5mL of media while control two had 5.950 mL of media and 0.050 of D.aquaticus. 


September 22, 2022

Twenty-four hour reading of the concentrations to see the growth 


Set A 

Set B 

Concentration 1 

0.01

0.02

Concentration 2

0.02

0.02

Concentration 3

0.03

0.04

Concentration 4

0.05

0.03

Concentration 5

0.02

0.02



I was not there for the forty-eight hour reading so I do not have the results for it yet. I will include those in the beginning of next week’s blog post. From the twenty-four hour reading, there was minimal growth but these results are not as significant as the forty-eight hour reading results.

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