Week 4 - October 7, 2022

 Thursday, October 6, 2022


Well Plate Concentrations 

Run 

TGY (Media) Concentration 

Antibiotic Concentration  

Concentration 1

299.10 

0.9

Concentration 2 

299.18

0.825

Concentration 3 

299.25

0.75

Concentration 4

299.33

0.675

Concentration 5 

299.4

0.60

Concentration 6 

299.48

0.525

Concentration 7

299.95

0.45

Concentration 8 

299.63

0.375

Concentration 9 

299.70

0.30

Concentration 10 

299.78

0.225


I observed a well plate preparation. This was the first time that I was able to see how you are supposed to work under the hood. From oberving, I learned that everything has to be wiped down before it enters the hood. Once it enters, it should not leave. If it does, it must be wiped down again with ethanol before re-entering. If your hands leave the hood, they must be sprayed with ethanol. 



Friday October 7, 2022

Four Way Streak: 

  • Put on gloves and make sure the gas is turned on

  • Make sure you have your bunsen burner ready along with your cultured media, inoculation ring, and plates

  • Vortex the cultured media 

  • Turn on bunsen burner 

  • Heat up inoculation ring over the flame until the ring is red hot

    • Wait about 30 seconds for the ring to cool off

      • If it is too hot, it will kill the bacteria 

      • You can test to see if it is too hot by touching the ring to the inside of the tube where your cultured media is. If it is too hot, it will sizzle. 

  • Dip the inoculation ring into the cultured media to get a bit of media

    • Quickly close the bottle of the cultured media so it is not exposed for too long

  • Start on the streaks


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